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Development and application of an ELISA for the detection of duck antibodies against Riemerella anatipestifer antigens in egg yolk of vaccinees and in serum of their offspring

Authors :
B. Schlatterer
L. Lobbedey
Source :
Journal of Veterinary Medicine Series B. 50:81-85
Publication Year :
2003
Publisher :
Wiley, 2003.

Abstract

A direct and an indirect antibody enzyme-linked immunosorbent assay for duck yolk IgY and duck serum IgY was developed and tested on egg yolk and serum of ducks vaccinated with Riemerella anatipestifer (Ra). Tests were performed either with primary antibodies labelled with horseradish peroxidase or with alkaline phosphatase-labelled secondary antibodies reacting with specifically bound rabbit anti-duck IgY antibodies, respectively. Ra-specific IgYs in egg yolk from three ducks increased rapidly at day 8 after the first of two vaccinations. In two ducks, the IgY titre persisted on a high plateau for 3 months. The concentration of Ra-specific IgYs in the serum of the progeny of vaccinees decreased between day 3 and day 10 after hatching. The fraction of total IgYs decreased less but also significantly. It was shown that antibodies were vertically transmitted and therefore protect offspring against Ra infection at least during the first week after hatching. The test design with anti-IgY rabbit antibodies is further suitable to detect other specific antibodies if respective antigens were fixed on solid phases.

Details

ISSN :
14390450 and 09311793
Volume :
50
Database :
OpenAIRE
Journal :
Journal of Veterinary Medicine Series B
Accession number :
edsair.doi.dedup.....003f1a7e05ff272f6a2095aa32f55d35
Full Text :
https://doi.org/10.1046/j.1439-0450.2003.00624.x