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Luteinizing hormone receptors are expressed in rat myenteric neurons and mediate neuronal loss
- Source :
- Autonomic Neuroscience. 193:104-107
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Background Clinical observations have suggested repeated gonadotropin-releasing hormone (GnRH) exposure to cause intestinal dysfunction and loss of enteric neurons. This has been further studied and confirmed in a rat in vivo model involving iterated GnRH treatments. Mechanisms behind are enigmatic since no GnRH receptors are found to be expressed in enteric neurons neither in man nor rat. Both species, however, harbor substantial subpopulations of luteinizing hormone (LH) receptor-immunoreactive myenteric neurons which suggests that intestinal GnRH-induced neuropathy may be mediated by LH release. Aims To reveal if exposures of GnRH or LH to rat myenteric neurons in vitro cause neuronal loss. Methods Primary cultured adult rat myenteric neurons were exposed to single or repeated treatments of the GnRH analog buserelin or the LH analog lutrotropin alpha, and neuronal survival was determined by cell counting. Possible presence of GnRH- or LH receptor -immunoreactive neurons was determined by immunocytochemistry. Results Exposure to the LH, but not the GnRH, analog caused significantly reduced neuronal survival. LH, but not GnRH, receptors were found to be expressed on cultured myenteric neurons. Conclusion Myenteric neurons express LH receptors in vitro and LH exposure causes reduced neuronal survival. This suggests that GnRH-induced enteric neuropathy in vivo is mediated by way of LH release and activation of enteric neuronal LH receptors.
- Subjects :
- Male
endocrine system
medicine.medical_specialty
Cell Survival
Immunocytochemistry
Myenteric Plexus
Cell Count
Gonadotropin-releasing hormone
Biology
Buserelin
Gonadotropin-Releasing Hormone
Rats, Sprague-Dawley
Cellular and Molecular Neuroscience
Ileum
Internal medicine
medicine
Animals
Receptor
Cells, Cultured
Myenteric plexus
Neurons
Dose-Response Relationship, Drug
Endocrine and Autonomic Systems
Enteric neuropathy
luteinizing hormone/choriogonadotropin receptor
Luteinizing Hormone
Receptors, LH
medicine.disease
Immunohistochemistry
Hormones
Endocrinology
nervous system
Female
Neurology (clinical)
Luteinizing hormone
Receptors, LHRH
hormones, hormone substitutes, and hormone antagonists
medicine.drug
Subjects
Details
- ISSN :
- 15660702
- Volume :
- 193
- Database :
- OpenAIRE
- Journal :
- Autonomic Neuroscience
- Accession number :
- edsair.doi.dedup.....003ddc911f3f59f21e371d0eb3b2b899