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Pseudomonas lurida sp. nov., a fluorescent species associated with the phyllosphere of grasses

Authors :
Jean-Marie Meyer
Undine Behrendt
Andreas Ulrich
Cathrin Spröer
Peter Schumann
Leibniz-Zentrum für Agrarlandschaftsforschung (ZALF)
Leibniz Association
German Collection of Microorganismes and Cell Cultures (DSMZ)
DSMZ
Génétique moléculaire, génomique, microbiologie (GMGM)
Université Louis Pasteur - Strasbourg I-Centre National de la Recherche Scientifique (CNRS)
Source :
International Journal of Systematic and Evolutionary Microbiology, International Journal of Systematic and Evolutionary Microbiology, Microbiology Society, 2007, 57 (Pt 5), pp.979-85. ⟨10.1099/ijs.0.64793-0⟩
Publication Year :
2007

Abstract

The taxonomic position of a group of fluorescent pseudomonad strains isolated from the phyllosphere of grasses was investigated through a polyphasic approach. Riboprinting analysis revealed highly similar patterns for the investigated strains which supported, together with the agreement of many phenotypic characteristics, their affiliation to the same species. A comparison of 16S rRNA gene sequences of strain P 513/18T, a representative strain from the grass isolates, revealed that it was affiliated to the cluster of the ‘Pseudomonas fluorescens group’, with Pseudomonas costantinii as the closest phylogenetic neighbour. However, DNA–DNA hybridization showed a clear demarcation at the species level between strain P 513/18T and P. costantinii. Furthermore, a comparison of riboprint patterns with Pseudomonas species clustering next to the novel grass isolates on the basis of 16S rRNA gene sequences supported their separate species status at the phylogenetic level. Based on phenotypic features, the novel isolates could also be differentiated from the other fluorescent Pseudomonas species that share positive arginine dihydrolase and oxidase reactions. As a consequence of these phenotypic and phylogenetic analyses, the isolates from the grass pyllosphere represent a novel species for which the name Pseudomonas lurida sp. nov. is proposed. The type strain is P 513/18T (=DSM 15835T=LMG 21995T).

Details

ISSN :
14665026 and 14665034
Volume :
57
Issue :
Pt 5
Database :
OpenAIRE
Journal :
International journal of systematic and evolutionary microbiology
Accession number :
edsair.doi.dedup.....0036ea76e19cf0fa86a322729551fbfd
Full Text :
https://doi.org/10.1099/ijs.0.64793-0⟩