Back to Search Start Over

Localization of l-DOPA uptake and decarboxylating neuronal structures in the cat brain using dopamine immunohistochemistry

Localization of l-DOPA uptake and decarboxylating neuronal structures in the cat brain using dopamine immunohistochemistry

Authors :
Ryohachi Arai
Jean-Marc Pequignot
Ikuko Nagatsu
Keiko Ogawa
Kunio Kitahama
Michel Geffard
Silvia Araneda
Physiologie intégrative, cellulaire et moléculaire (PICM)
Centre National de la Recherche Scientifique (CNRS)-Université Claude Bernard Lyon 1 (UCBL)
Université de Lyon-Université de Lyon
Laboratoire de physique des interactions ondes matières (LPIOM)
Université Sciences et Technologies - Bordeaux 1-École Nationale Supérieure de Chimie et de Physique de Bordeaux (ENSCPB)-Centre National de la Recherche Scientifique (CNRS)
Laboratoire de l'intégration, du matériau au système (IMS)
Université Sciences et Technologies - Bordeaux 1-Institut Polytechnique de Bordeaux-Centre National de la Recherche Scientifique (CNRS)
Department of Anatomy
Shiga University of Medical Science
Physio-pathologie des réseaux neuronaux du cycle veille-sommeil
Fujita Health University School of Medicine
Source :
Brain Research, Brain Research, Elsevier, 2007, 1167, pp.56-70. ⟨10.1016/j.brainres.2007.05.081⟩
Publication Year :
2007
Publisher :
Elsevier BV, 2007.

Abstract

International audience; The present study examined dopamine-immunoreactive neuronal structures using immunohistochemistry in conjunction with an anti-dopamine antiserum, following injection of l-3,4-dihydroxyphenylalanine (L-DOPA) with or without an inhibitor of monoamine oxidase (Pargyline) in the cat brain. L-DOPA injection made it possible to detect dopamine immunoreactivity in presumptive serotonergic and noradrenergic cell bodies and axons. Weak to moderate dopamine immunoreactivity was observed in non-aminergic cells (possibly so-called "D" cells containing aromatic L-amino acid decarboxylase (AADC)) in several hypothalamic, midbrain, pontine and medullary nuclei. Intense dopamine immunoreactivity became visible in a large number of cells and axons (possibly containing AADC) with wide distribution in the brain following administration of L-DOPA with Pargyline. AADC is most likely active in cells and axons that take up L-DOPA, where it decarboxylates the L-DOPA to dopamine. However, newly synthesized dopamine in such cells is rapidly oxidized by monoamine oxidase.

Details

ISSN :
00068993
Volume :
1167
Database :
OpenAIRE
Journal :
Brain Research
Accession number :
edsair.doi.dedup.....0034f53d55ee30fe60304e7e8bffebdf
Full Text :
https://doi.org/10.1016/j.brainres.2007.05.081