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Localization of l-DOPA uptake and decarboxylating neuronal structures in the cat brain using dopamine immunohistochemistry
Localization of l-DOPA uptake and decarboxylating neuronal structures in the cat brain using dopamine immunohistochemistry
- Source :
- Brain Research, Brain Research, Elsevier, 2007, 1167, pp.56-70. ⟨10.1016/j.brainres.2007.05.081⟩
- Publication Year :
- 2007
- Publisher :
- Elsevier BV, 2007.
-
Abstract
- International audience; The present study examined dopamine-immunoreactive neuronal structures using immunohistochemistry in conjunction with an anti-dopamine antiserum, following injection of l-3,4-dihydroxyphenylalanine (L-DOPA) with or without an inhibitor of monoamine oxidase (Pargyline) in the cat brain. L-DOPA injection made it possible to detect dopamine immunoreactivity in presumptive serotonergic and noradrenergic cell bodies and axons. Weak to moderate dopamine immunoreactivity was observed in non-aminergic cells (possibly so-called "D" cells containing aromatic L-amino acid decarboxylase (AADC)) in several hypothalamic, midbrain, pontine and medullary nuclei. Intense dopamine immunoreactivity became visible in a large number of cells and axons (possibly containing AADC) with wide distribution in the brain following administration of L-DOPA with Pargyline. AADC is most likely active in cells and axons that take up L-DOPA, where it decarboxylates the L-DOPA to dopamine. However, newly synthesized dopamine in such cells is rapidly oxidized by monoamine oxidase.
- Subjects :
- Dopamine
MESH: Neurons
Levodopa
Norepinephrine
chemistry.chemical_compound
0302 clinical medicine
MESH: Animals
Neurotransmitter
Neurons
MESH: Levodopa
0303 health sciences
Aromatic L-amino acid decarboxylase
Chemistry
General Neuroscience
Brain
Neurochemistry
Immunohistochemistry
3. Good health
Aromatic-L-Amino-Acid Decarboxylases
MESH: Aromatic-L-Amino-Acid Decarboxylases
MESH: Cats
medicine.drug
Serotonin
MESH: Axons
[SDV.OT]Life Sciences [q-bio]/Other [q-bio.OT]
medicine.medical_specialty
Monoamine Oxidase Inhibitors
Monoamine oxidase
MESH: Monoamine Oxidase Inhibitors
MESH: Dopamine
MESH: Pargyline
Serotonergic
MESH: Neurochemistry
MESH: Brain
03 medical and health sciences
MESH: Norepinephrine
Internal medicine
medicine
Animals
Molecular Biology
030304 developmental biology
MESH: Immunohistochemistry
Pargyline
Axons
Endocrinology
Cats
Catecholamine
MESH: Serotonin
Neurology (clinical)
030217 neurology & neurosurgery
Developmental Biology
Subjects
Details
- ISSN :
- 00068993
- Volume :
- 1167
- Database :
- OpenAIRE
- Journal :
- Brain Research
- Accession number :
- edsair.doi.dedup.....0034f53d55ee30fe60304e7e8bffebdf
- Full Text :
- https://doi.org/10.1016/j.brainres.2007.05.081