Back to Search
Start Over
Identification and expression analysis of GPAT family genes during early development of Xenopus laevis
- Source :
- Gene expression patterns : GEP. 12(7-8)
- Publication Year :
- 2011
-
Abstract
- Production of lysophosphatidic acid (LPA) is the first step in the de novo pathway for glycerolipid biosynthesis, which is mainly catalyzed by the glycerol-3-phosphate acyltransferases (GPATs; EC2.3.1.15). DHAPAT (EC2.3.1.42) also contributes in a minor way, using dihydroxyacetone phosphate as substrate. Final products and intermediates of the glycerolipid synthesis pathway are the main structural components of cellular membranes, and provide signalling molecules that regulate diverse biological processes, including cell proliferation, differentiation and growth. Here we identified the four orthologs of the mammalian GPATs (1–4) and DHAPAT in Xenopus, including a novel, short variant of GPAT2, and analyzed their expression pattern during embryonic development. Xenopus GPAT1/2 localized to mitochondria, while GPAT3/4 associated with the endoplasmic reticulum. All are similarly expressed in the early embryonic nervous system. A more tissue specific pattern emerges during organogenesis, including liver expression for GPAT1/4, and testis expression for GPAT2. All acyltransferases were expressed in kidney, though GPAT3 was excluded from the pronephric ducts. Our results suggest important roles of GPATs and DHAPAT during early organogenesis.
- Subjects :
- Male
Embryo, Nonmammalian
Transcription, Genetic
Organogenesis
Xenopus
Embryonic Development
Xenopus Proteins
Kidney
Gene Expression Regulation, Enzymologic
chemistry.chemical_compound
Xenopus laevis
Lysophosphatidic acid
Chlorocebus aethiops
Testis
Genetics
Animals
Molecular Biology
Gene
Dihydroxyacetone phosphate
biology
Endoplasmic reticulum
Gene Expression Regulation, Developmental
biology.organism_classification
Embryonic stem cell
Cell biology
chemistry
Biochemistry
Liver
Acyltransferases
Organ Specificity
Larva
COS Cells
Glycerol-3-Phosphate O-Acyltransferase
Transcriptome
Developmental Biology
Subjects
Details
- ISSN :
- 18727298
- Volume :
- 12
- Issue :
- 7-8
- Database :
- OpenAIRE
- Journal :
- Gene expression patterns : GEP
- Accession number :
- edsair.doi.dedup.....00251c2bbe9a01e7147da2c8d73b7573