Additional file 1 of PCR-based genotyping assays to detect germline APC variant associated with hereditary gastrointestinal polyposis in Jack Russell terriers

Additional file 1: Figure S1. PCR-RFLP assay conducted to determine the optimal digestion time. Acrylamide gel electrophoresis of MseI-digested PCR products amplified from synthetic wild-type and mutant DNA (A), and blood-derived DNA samples of a carrier and a non-carrier of germline APC variant (B). PCR products amplified from each sample were digested with MseI for 0, 1, 2, 4, 8, 12, and 24 h.