Glucose oxidase production byPenicillium variabileP16: effect of medium composition

The effect of medium composition on glucose oxidase (GOD) production by the strain Penicillium variabile P16 was studied in shake culture. All the experiments were performed in a medium containing glucose and sodium nitrate as carbon and nitrogen sources, respectively. Addition of Fe 2+ to the medium was important to stimulate GOD production. Calcium carbonate was extremely important in maintaining the medium at a pH high enough so that the enzyme was not inactivated. The time course of growth and enzyme production by P. variabile P16 in the optimized medium is reported