Nuclear Changes in Rous Sarcoma Cells Cultivated in vitro

Various authors (Borrel,1 Roskin,2 Lipschutz,3 Fischer,4 Zweibaum5) have indicated that the structure of Rous sarcoma cells shows certain peculiarities, which differentiate them from normal mesenchyme cells.These indications are very fragmentary and there is little agreement between the various descriptions. The morphological features of the Rous sarcoma cell are still not sufficiently known.The structural peculiarities of the Rous sarcoma cells appear with great clarity and they are easily detectable in pure culture of sarcoma cells. Our studies of cell strains, cultured without addition of normal tissue revealed that the sarcoma cells in vitro constantly show profound morphological alterations of their cytoplasma and nuclei, often associated with marked, indeed extraordinary hypertrophy of the entire cells.In this note we report on the changes taking place in the nuclei of the sarcoma cells. The studies were made on cultures (preparations in toto and serial sections) fixed in Carnoy and stained with Gie...