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Highly sensitive electrochemiluminescent immunoassay for neuron-specific enolase amplified by single-walled carbon nanohorns and enzymatic biocatalytic precipitation
- Source :
- Journal of Electroanalytical Chemistry. 818:257-264
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- A novel amplified electrochemiluminescence (ECL) immunoassay based on single-walled carbon nanohorns (SWCNHs) labels and enzymatic biocatalytic precipitation (BCP) was proposed for the detection of neuron-specific enolase (NSE) for the first time. In this system, the dimercaptosuccinic acid stabilized CdTe (DMSA-CdTe) quantum dots (QDs) as ECL emitter showed excellent ECL behavior and then employed as the NSE antibody (Ab1) immobilization film for the subsequent sandwich-type Ab1-Ag affinity interactions. Improved sensitivity was achieved through using the SWCNHs effectively to expand the amount of secondary antibody (Ab2) and horseradish peroxidase (HRP) loading. In addition to the much enhanced steric hindrance, compared with the single HRP-Ab2, the presence of plentiful HRP would further stimulate the BCP onto the electrode surface for signal amplification, concomitant to the BCP products absorption that lowered ECL intensity. As a result of the multisignal amplification in this ECL immunosensor, it possessed excellent analytical performance. The target NSE could be detected from 1 × 10−9 g L−1 to 1 × 10−3 g L−1 with a detection limit of 4.4 × 10−10 g L−1.
- Subjects :
- Detection limit
endocrine system
Chromatography
medicine.diagnostic_test
biology
Chemistry
General Chemical Engineering
Enolase
02 engineering and technology
Single-walled carbon nanohorn
010402 general chemistry
021001 nanoscience & nanotechnology
01 natural sciences
Horseradish peroxidase
Primary and secondary antibodies
0104 chemical sciences
Analytical Chemistry
Immunoassay
Electrode
Electrochemistry
medicine
biology.protein
Electrochemiluminescence
0210 nano-technology
Subjects
Details
- ISSN :
- 15726657
- Volume :
- 818
- Database :
- OpenAIRE
- Journal :
- Journal of Electroanalytical Chemistry
- Accession number :
- edsair.doi...........ff8e3dfc33739e1e6362deef139aef80