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Supplementary Materials for the manuscript entitled "Transcriptomic and metabolomic responses of rice plants to Cnaphalocrocis medinalis caterpillar infestation" submitted to "Insects". Supplementary figures Fig. S1. Design of the rice leafroller feeding experiments. The 2nd leaf of a five-week-old Minghui 63 rice plant was enclosed in a Parafilm bag. At staggered intervals, one rice leafroller C. medinalis caterpillar was added to each bag. Leaf tissue was harvested after 0 to 24 h of leafroller feeding. Harvested samples were immediately frozen in liquid nitrogen and stored at −80 °C for further processing. Fig. S2. Phenotypes of rice leaves infested by C. medinalis at different time points. Fig. S3. Comparison of mRNA expression levels detected by RNA-seq and qRT-PCR. All qRT-PCR data were normalized against that of the housekeeping gene ubiquitin 5. Values are means ± SE; n = 4 for RNA-seq and n = 3 for qRT-PCR. Specific information on these genes is provided in Table S1. Fig. S4. Heat map of the expression of calcium ion sensor genes induced by C. medinalis feeding at different time points. Asterisks indicate a significant difference in the expression of genes at different time points relative to control (0 h): * padj < 0.05, **padj < 0.01 via the Benjamini and Hochberg adjustment method. Fig. S5. Heat map of the expression of DEGs involved in mitogen-activated protein kinase (MAPK)-WRKY pathways in response to C. medinalis feeding at different time points. Asterisks indicate a significant difference of the expression of genes in different time points relative to control (0 h): * padj < 0.05, **padj < 0.01 via the Benjamini and Hochberg adjustment method. Supplementary tables Table S1. Genes and primer pairs used for quantitative real-time PCR. Table S2. Summary for the transcriptome dataset. Table S3. DEGs in rice leaves in response to C. medinalis feeding at different time points. Table S4. Differentially expressed transcription factors and transcriptional regulator in rice leaves in response to C. medinalis feeding. Table S5. GO enrichment analysis of the total DEGs of rice leaves in response to C. medinalis feeding at different time points. Table S6. KEGG enrichment analysis of the upregulated and downregulated DEGs in rice leaves in response to C. medinalis feeding at different time points. Table S7. Clustering analyses of DEGs in rice leaves in response to C. medinalis feeding at least one time point. Table S8. KEGG analyses of DEGs belong to different clusters. Table S9. Orthologous Arabidopsis and rice genes used for Hormonometer analysis.