The inhibition of lactate dehydrogenase by salicylate

1. 1. The inhibition of bands 5 and 1 isoenzymes of porcine lactate dehydrogenase by salicylate was investigated kinetically. 2. 2. These studies indicated that up to two molecules of salicylate could combine with the active site of the free band 5 isoenzyme, and that one of the two salicylate molecules competes with its AMP binding site. 3. 3. It is concluded that salicylate competes with the AMP moiety of NAD+ in this enzyme.