Analysis of variability in partial sequences of genomes of Tobacco yellow dwarf virus isolates

The movement protein coding region of six isolates of Tobacco yellow dwarf virus (TYDV), collected over a 30 year period from various regions throughout Australia, was analysed using PCR and DNA sequencing. Four isolates of Bean summer death virus, considered a synonym of TYDV, were also analysed. Phylogenetic analysis showed that all 10 isolates were greater than 95% homologous to the published TYDV sequence (GenBank accession number M81103). The weed Raphanus raphanistrum was identified as an alternative host of TYDV for the first time.