Estrogen Deficiency Induces Bone Loss in Mice Lacking ITAM Adapter Proteins, DAP12 and FcRg (89.31)

DAP12 and or FcRγ ITAM signals are required during normal osteoclast (OC) development in vivo and in vitro. To determine whether ITAM deficient mice are resistant to bone loss induced by estrogen deficiency, we performed ovariectomy (OVX) or SHAM surgery on 10–12 week female DAP12−/−, FcRγ−/−, DAP12−/−FcRγ−/− and control C57BL/6 (B6) mice. MicroCT of the distal femur showed statistically significant losses of trabecular bone in DAP12−/−FcRγ−/−, DAP12−/−, FcRγ−/− and B6 OVX groups compared to SHAM groups, BV/TV dropping from 56% to 35% (p