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Extraction of Damba Black Soybean Gene by Means of Magnetic Nanoparticles and Investigation of Aluminum Tolerance
- Source :
- Journal of Biobased Materials and Bioenergy. 15:156-162
- Publication Year :
- 2021
- Publisher :
- American Scientific Publishers, 2021.
-
Abstract
- The aluminum tolerance of Tamba black soybean (Glycine max cv. Tamba) is closely related to organic acid secretion mechanisms. The gene responsible for this tolerance in this variety (GmFER84) is extracted from lysates of soybean root tips using silylated Fe3O4 nanomagnetic beads. GmFER84 (Glycine max XP 003540203.1) is a stable protein. Tobacco genetically transformed with GmFER84 using an Agrobacterium-mediated transformation was tested for aluminum tolerance. Citrate synthase and citric acid secretion in the roots of transgenic tobacco prove to be significantly higher than those of wild tobacco, and the antioxidant properties of transgenic tobacco are also substantially increased. Research on GmFER84 may enable further agronomic development.
- Subjects :
- 0106 biological sciences
Materials science
Renewable Energy, Sustainability and the Environment
Extraction (chemistry)
chemistry.chemical_element
Bioengineering
02 engineering and technology
021001 nanoscience & nanotechnology
01 natural sciences
Biomaterials
chemistry
Aluminium
Magnetic nanoparticles
0210 nano-technology
010606 plant biology & botany
Nuclear chemistry
Subjects
Details
- ISSN :
- 15566560
- Volume :
- 15
- Database :
- OpenAIRE
- Journal :
- Journal of Biobased Materials and Bioenergy
- Accession number :
- edsair.doi...........fd89b94a9fad82a09597974557633d7f