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Epigallocatechin-3-gallate enhances tomato resistance to tobacco mosaic virus by modulating RBOH1-dependent H2O2 signaling
- Source :
- Plant Physiology and Biochemistry. 150:263-269
- Publication Year :
- 2020
- Publisher :
- Elsevier BV, 2020.
-
Abstract
- Tobacco mosaic virus (TMV) is one of the most damaging plant viruses from an economic and research point of view. Epigallocatechin-3-Gallate (EGCG), a flavonoid type secondary metabolite can selectively improve plant defense against pathogens; however, the effect of EGCG on plant defense against TMV and the underlying mechanism(s) remain elusive. In this study, exogenous EGCG application increased plant resistance to TMV as revealed by significantly decreased transcript levels of TMV-coat protein (CP) in tomato leaves. A time-course of H2O2 concentrations in tomato leaves showed that TMV inoculation rapidly increased the H2O2 accumulation, reaching its peak at 3 days post-inoculation (dpi) which remained the highest until 6 dpi. However, the combined treatment of EGCG and TMV remarkably decreased the concentrations of H2O2 at 3 and 6 dpi. Meanwhile, the transcript levels of RESPIRATORY BURST OXIDASE HOMOLOG 1 (SlRBOH1) were significantly increased by either EGCG or TMV inoculation, but the EGCG treatment along with TMV caused a further upregulation in the SlRBOH1 transcripts compared with that in only TMV-inoculated plants. Chemical scavenging of H2O2 or silencing SlRBOH1 both compromised the EGCG-induced enhanced resistance to TMV. Furthermore, EGCG-induced elevation in the activity of antioxidant enzymes was abolished by SlRBOH1 silencing, suggesting that EGCG enhanced defense against TMV by increasing the antioxidant enzyme activity via RBOH1-dependent H2O2 signaling. Taken together, our results suggest that EGCG functioned to maintain a delicate balance between ROS signaling and ROS scavenging via RBOH1, which enhanced tomato resistance to TMV.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Antioxidant
Physiology
viruses
medicine.medical_treatment
Plant Science
complex mixtures
01 natural sciences
03 medical and health sciences
Downregulation and upregulation
Plant virus
Genetics
Tobacco mosaic virus
Plant defense against herbivory
medicine
Gene silencing
heterocyclic compounds
chemistry.chemical_classification
Reactive oxygen species
biology
fungi
food and beverages
Enzyme assay
Cell biology
030104 developmental biology
chemistry
biology.protein
010606 plant biology & botany
Subjects
Details
- ISSN :
- 09819428
- Volume :
- 150
- Database :
- OpenAIRE
- Journal :
- Plant Physiology and Biochemistry
- Accession number :
- edsair.doi...........fd6ec8c5451faff0b3bb951e9ef3a622