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B-type natriuretic peptide inhibits angiotensin II-induced proliferation and migration of pulmonary arterial smooth muscle cells
- Source :
- Pediatric Pulmonology. 49:734-744
- Publication Year :
- 2013
- Publisher :
- Wiley, 2013.
-
Abstract
- Pulmonary vascular remodeling, characterized by disordered proliferation and migration of pulmonary arterial smooth muscle cells (PASMCs), is a pathognomonic feature of pulmonary arterial hypertension. Thus, pharmacologic strategy targeting on anti-proliferation and anti-migration of PASMCs may have therapeutic implications for PAH. Here we investigated the effects and underlying mechanisms of B-type natriuretic peptide (BNP) on angiotensin II (Ang II)-induced proliferation and migration of PASMCs. Proliferation and migration of PASMCs cultured from Wistar rats were induced by Ang II, with or without BNP treatment. In addition, potential underlying mechanisms including cell cycle progression, Ca(2+) overload, reactive oxygen species (ROS) production, signal transduction of MAPK and Akt, and the cGMP/PKG pathway were examined. We found that BNP inhibited Ang II-induced PASMCs proliferation and migration dose dependently. BNP could also arrest the cell cycle progression in the G0/G1-phase. In addition, BNP attenuated intracellular calcium overload caused by Ang II. Moreover, Ang II-induced ROS production was mitigated by BNP, with associated down-regulation of NAD(P)H oxidase 1 (Nox1) and reduced mitochondrial ROS production. Finally, Ang II-activated MAPKs and Akt were also counteracted by BNP. Of note, all these effects of BNP were abolished by a PKG inhibitor (Rp-8-Br-PET-cGMPS). In conclusion, BNP inhibits Ang II-induced PASMCs proliferation and migration. These effects are potentially mediated by decreased calcium influx, reduced ROS production by Nox1 and mitochondria, and down-regulation of MAPK and Akt signal transduction, through the cGMP/PKG pathway. Therefore, this study implicates that BNP may have a therapeutic role in pulmonary vascular remodeling.
- Subjects :
- Pulmonary and Respiratory Medicine
Mitochondrial ROS
MAPK/ERK pathway
medicine.medical_specialty
business.industry
medicine.drug_class
Angiotensin II
Calcium in biology
Endocrinology
NOX1
Internal medicine
Pediatrics, Perinatology and Child Health
cardiovascular system
medicine
Natriuretic peptide
Signal transduction
business
Protein kinase B
hormones, hormone substitutes, and hormone antagonists
Subjects
Details
- ISSN :
- 87556863
- Volume :
- 49
- Database :
- OpenAIRE
- Journal :
- Pediatric Pulmonology
- Accession number :
- edsair.doi...........fc783ccd97082e4e179c8dfe48b07130