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The Circular RNA ciRs-126 Predicts Survival in Critically Ill Patients With Acute Kidney Injury
- Source :
- Kidney International Reports. 3:1144-1152
- Publication Year :
- 2018
- Publisher :
- Elsevier BV, 2018.
-
Abstract
- Introduction Circular RNAs (circRNAs) have recently been described as novel noncoding regulators of gene expression. They might have an impact on microRNA expression by their sponging activity. The detectability in blood of these RNA transcripts has been demonstrated in patients with cancer and cardiovascular disease. We tested the hypothesis that circulating circRNAs in blood of critically ill patients with acute kidney injury (AKI) at inception of renal replacement therapy may also be dysregulated and associated with patient survival. Methods We performed a global circRNA expression analysis using RNA isolated from blood of patients with AKI as well as controls. This global screen revealed several dysregulated circRNAs in patients with AKI. Most highly increased circRNA-array−based transcripts as well as expression of the circRNA target miR-126-5p were confirmed in blood of 109 patients with AKI, 30 age-matched healthy controls, 25 critically ill non-AKI patients, and 20 patients on maintenance hemodialysis by quantitative real-time polymerase chain reaction. Results Circulating concentrations of 3 novel circRNAs were amplified in blood of patients with AKI and in controls. Circular RNA sponge of miR-126 (or ciRs-126) was most highly altered compared to healthy controls and disease controls (fold change of 52.1). ciRs-126 was shown to bioinformatically sponge miR-126-5p, which was found to be highly suppressed in AKI patients and hypoxic endothelial cells. Cox regression and Kaplan−Meier curve analysis revealed ciRs-126 as an independent predictor of 28-day survival (P Conclusion Circulating concentrations of circRNAs in patients with AKI are detectable. ciRs-126 may potentially sponge miR-126-5p and acts as a predictor of mortality in this patient cohort.
- Subjects :
- 0301 basic medicine
Oncology
medicine.medical_specialty
Proportional hazards model
business.industry
medicine.medical_treatment
Acute kidney injury
Cancer
RNA
medicine.disease
03 medical and health sciences
030104 developmental biology
0302 clinical medicine
Nephrology
Circular RNA
030220 oncology & carcinogenesis
Internal medicine
microRNA
Gene expression
medicine
Renal replacement therapy
business
Subjects
Details
- ISSN :
- 24680249
- Volume :
- 3
- Database :
- OpenAIRE
- Journal :
- Kidney International Reports
- Accession number :
- edsair.doi...........fbd9795d2d35635c15e075b121185760