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Improvement of co-occurrence matrix calculation and collagen fibers orientation estimation
- Source :
- SPIE Proceedings.
- Publication Year :
- 2017
- Publisher :
- SPIE, 2017.
-
Abstract
- Gray-level co-occurrence matrix (GLCM) is a statistical method widely used to characterize images and specifically, for Second Harmonic Generation (SHG) collagen images characterization. This method takes into account the spatial relationship between the image pixels, at specific angle. It is usually calculated for four orientations, at specific distances. Over these matrix, a textural feature function is calculated. Often, results of different orientations are compared or averaged to get a unique statistic parameter. In the present report, we will demonstrate the error that bring with this methodology, and following, we offer the correction formula. Preferred orientation of SHG images is proposed as structural property to characterize biological samples. For example, for determining the parallelism grade of collagen fibers regarding the ovarian epithelium. Here, we present a robust method to calculate this parameter, based on the two-dimensional Fourier transform. Finally, we show how these two elements help improve the discrimination between normal and pathological ovarian tissues.
- Subjects :
- Pixel
business.industry
Orientation (computer vision)
Quantitative Biology::Tissues and Organs
Statistical parameter
Texture (geology)
symbols.namesake
Matrix (mathematics)
Co-occurrence matrix
Fourier transform
Feature (computer vision)
Computer Science::Computer Vision and Pattern Recognition
symbols
Computer vision
Artificial intelligence
business
Biological system
Mathematics
Subjects
Details
- ISSN :
- 0277786X
- Database :
- OpenAIRE
- Journal :
- SPIE Proceedings
- Accession number :
- edsair.doi...........fba5aee8b055b02249d3b4748a0b758c
- Full Text :
- https://doi.org/10.1117/12.2256721