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Silencing of OBP genes: Generation of loss-of-function mutants of PBP by genome editing
- Publication Year :
- 2020
- Publisher :
- Elsevier, 2020.
-
Abstract
- Pheromone binding proteins (PBPs) are small soluble proteins (about 15kDa) that play striking roles in the detection of sex pheromones in insects. Many studies including structural analysis, binding simulation, and in vitro assays have been performed to clarify the modes of action of PBPs. Although these studies have provided valuable contributions toward the understanding of which key amino acid components contribute to the correct folding of PBPs and their binding affinities to sex pheromones, the functional characteristics of PBPs in the natural environment is still obscure. Recent developments in genome editing have begun to enable the functional examination of PBPs in in vivo. Among insect PBPs, BmPBP1 is one of the most well-characterized, there being rich understanding of its structure, biochemical analysis, binding affinity, localization, and the relationship between the type of olfactory receptors and its expression. A recent study has shown that BmPBP1 contributes sensitivity, but not selectivity of sex pheromone detection in the silkmoth Bombyx mori. In this chapter, based on a current report of the functional characterization of BmPBP1 using genome editing, we provide one example of a useful analytical method to clarify the functional role of PBP in vivo.
- Subjects :
- 0303 health sciences
Transcription activator-like effector nuclease
030303 biophysics
Mutant
Computational biology
biochemical phenomena, metabolism, and nutrition
Biology
03 medical and health sciences
Genome editing
Sex pheromone
polycyclic compounds
Pheromone binding
Pheromone binding protein
Gene
Loss function
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........fb41219c8a62a1958520026f4266d0db