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Determination of glucan phosphorylation using heteronuclear1H,13C double and1H,13C,31P triple-resonance NMR spectra
- Source :
- Magnetic Resonance in Chemistry. 51:655-661
- Publication Year :
- 2013
- Publisher :
- Wiley, 2013.
-
Abstract
- Phosphorylation and dephosphorylation of starch and glycogen are important for their physicochemical properties and also their physiological functions. It is therefore desirable to reliably determine the phosphorylation sites. Heteronuclear multidimensional NMR-spectroscopy is in principle a straightforward analytical approach even for complex carbohydrate molecules. With heterogeneous samples from natural sources, however, the task becomes more difficult because a full assignment of the resonances of the carbohydrates is impossible to obtain. Here, we show that the combination of heteronuclear (1) H,(13) C and (1) H,(13) C,(31) P techniques and information derived from spectra of a set of reference compounds can lead to an unambiguous determination of the phosphorylation sites even in heterogeneous samples.
- Subjects :
- inorganic chemicals
chemistry.chemical_classification
Chemistry
Resonance
macromolecular substances
General Chemistry
Spectral line
NMR spectra database
Dephosphorylation
Nuclear magnetic resonance
Heteronuclear molecule
Computational chemistry
Phosphorylation
Molecule
General Materials Science
Glucan
Subjects
Details
- ISSN :
- 07491581
- Volume :
- 51
- Database :
- OpenAIRE
- Journal :
- Magnetic Resonance in Chemistry
- Accession number :
- edsair.doi...........f9a710779312c91f5fd11775144e1c3c