Detection of the antibiotic resistance genes in Staphylococcus aureus isolated from human infections and bovine mastitis

The present study was carried out in an attempt to detect the distribution of antibiotic-resistant genes of Staphylococcus aureus isolates from human infections and bovine mastitis. mecA, msrA, msrB, aacA-D, tetK and tetM genes were selected in order to detect the distribution of antibiotic-resistant genes by multiplex PCR technique. According to the biochemical analysis and detection of 16S-rDNA by PCR method, 108 isolates of 300 human infections samples and 18 strains from 150 bovine mastitis milks were recognized as S. aureus. Distributions of antibiotic-resistant genes in human isolates were as follows: (85.18%) mecA, (46.29%) msrA, (49.07%) msrB, (33.33%) aacA-D, (80.50%) tetK and (66.66%) tetM and in bovine mastitis, isolates were seen to be ranging from: (22.22%) mecA, (66.66%) msrA, (77.77%) msrB, (33.33%) aacA-D, (55.55%) tetKand (50.00%) tetM, respectively. Results indicated that all S. aureus strains have one or more of the antibiotic-resistant genes. Also, multiplex PCR technique is a fast, practical and appropriate technique for determining antibiotic-resistant genes. Hence, it was possible that the treatment and the right antibiotics were used. Key words: Antibiotic-resistant genes, bovine mastitis, human infections, multiplex PCR,Staphylococcus aureus.