Chromatographic separation and characterization of the photoproduct of tris(l-cysteinesulfinato-N,S)cobaltate(III)

The product of photolysis of K 3 (+)-[Co( l -cysi-N,S) 3 ] ( 1 , l -cysi= l -cysteinesulfinate(2−) ion, cysi=NH 2 CH(COO − )CH 2 SO 2 − ) was separated by column chromatography on DEAE-Sephadex A-25. The main band contained [Co( l -cysi-N,S) 2 ( l -cysi-N,O)] 3− ( 2 ); linkage isomerization from sulfinato- S to sulfinato- O took place. This complex ( 2 ) is thermally unstable and reverts to the starting complex ( 1 ). An anion-exchange high-performance liquid chromatographic (HPLC) method was successfully applied to the kinetic study of this linkage isomerization reaction from 2 to 1 .