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Regulatory sequences of the Arabidopsis thaliana Rps19, a nuclear gene encoding mitochondrial ribosomal protein subunit, extend into the upstream gene
- Source :
- Journal of Plant Biochemistry and Biotechnology. 26:302-309
- Publication Year :
- 2016
- Publisher :
- Springer Science and Business Media LLC, 2016.
-
Abstract
- Genes encoding subunits of the mitochondrial ribosomal protein complex are distributed between the mitochondrial and nuclear genomes. In Arabidopsis thaliana only seven out of the nearly 70 genes coding for mitochondrial ribosomal subunit proteins are present in the mitochondrial genome. Nevertheless, these genes are co-ordinately expressed. Here, we present the first report of characterization of promoter of a plant mitochondrial ribosomal protein gene AtRps19 (At5g47320), a single copy nuclear gene in A. thaliana. Analysis of transgenic A. thaliana plants carrying seven different AtRps19 upstream fragments linked to the uidA reporter gene revealed that the 879 bp fragment containing the 5′ UTR and the intergenic region is capable of driving gene expression in most of the vegetative tissues. However, inclusion of 447 bp of the upstream gene sequences was essential for obtaining full expression in all tissues including anthers and pollen. Thus, we provide the first experimental proof of overlapping genes in plants. qRT-PCR analysis showed that uidA and native AtRps19 transcript levels were comparable in plants carrying the D4 (−676/+650) construct, indicating that the 1326 bp D4 fragment represents the native promoter of the AtRps19 gene. Comparison of AtRps19 promoter with the widely used CaMV35S promoter showed that AtRps19 promoter is capable of driving gene expression in all tissues including anthers and pollen but was less efficient than the CaMV35S promoter.
- Subjects :
- 0106 biological sciences
0301 basic medicine
Genetics
Reporter gene
Nuclear gene
food and beverages
Plant Science
Biology
MT-RNR1
01 natural sciences
03 medical and health sciences
030104 developmental biology
Ribosomal protein
Gene cluster
Agronomy and Crop Science
Gene
Overlapping gene
010606 plant biology & botany
Biotechnology
HSPA9
Subjects
Details
- ISSN :
- 09741275 and 09717811
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Journal of Plant Biochemistry and Biotechnology
- Accession number :
- edsair.doi...........f836152200eda635ffc3907fd48c1c5b