Additional file 7: Figure S3. of Influenza A virus infection impacts systemic microbiota dynamics and causes quantitative enteric dysbiosis

(A) rarefection plot: number of sequences is plotted against Chao1 estimator. Error bars indicate SE. (B) 2D PCoA of SI microbiota mock-treated (blue symbols) or IAV-infected mice (red symbols) at indicated time points post infection. (C) Mean UNIFRAC distances for the comparison of indicated experimental groups. Statistical significance was determined by two-tailed student’s T test. (D) 2D PCoA of SI microbiota mock-treated (blue symbols) or IAV-infected mice (red symbols) at indicated time points post infection. Each symbol represents one mouse. Each symbol type refers to an individual cage. (E) Shannon index, relative abundances of indicated bacterial classes determined by 16S rRNA gene NGS, and relative 16S/18S levels of animals 7 dpi are shown. Each symbol represents one animal. Each symbol type refers to an individual cage. (F) Relative abundance of total bacteria based on qPCR (16S/18S) normalized to median of mock samples is shown for individual mice and indicated time points treated with mock (black symbols) or indicated viruses (red symbols) for for H1N1 7 dpi (n = 8 per group) pH1N1 6 dpi (n = 5 per group). (G) Most abundant OTU on genus level. Median relative abundance (%) is depicted. (PDF 1956 kb)