Reduced H + channel activity disrupts pH homeostasis and calcification in coccolithophores at low ocean pH

Significance Coccolithophore calcification is a major ocean biogeochemical process. While this process is likely to be sensitive to acidification-driven changes in ocean carbonate chemistry, incomplete understanding of the underlying mechanisms and constraints is a major bottleneck in predicting ocean acidification effects on calcification. We report severe disruption of pH homeostasis linked to a loss of H + channel function in the coccolithophore Coccolithus braarudii acclimated to seawater pH values that are likely to be encountered currently in localized regions and more widely in future oceans. This disruption leads to specific defects in coccolith morphology. These findings provide mechanistic insight into how calcification in different coccolithophores is affected by changes in seawater carbonate chemistry.