The immunization of fowls against chicken-pox (Epithelioma contagiosum), by subcutaneous injection of virus

does not appear. First page follows. Introduction2 The studies reported herein consist of a series of experiments in the immunization of fowls against chicken-pox (Epithelioma contagiosum) by the subcutaneous injection of vaccine containing the lesion tissue removed from fowls affected with the disease. The first report of a successful attempt to immunize fowls against chicken-pox by such means was that of Manteufel(1) in 1910. He reported success in immunizing fowls by injecting into the circulation or under the skin a lymph prepared from scraping of lesions on the comb or mucous membranes of the head of diseased birds mixed with physiologic salt solution and heated in a water bath at 55° C for one hour. According to this investigator, chickens treated in this manner were immune to infection for from one and one-half to two years even though there was no visible reaction following vaccination. Marked curative value for this preparation was also claimed.