Human fibroblast behavior cultured in the eggshell membranes for tissue engineering applications

espanolLa membrana de la cascara del huevo ha sido considerada durante mucho tiempo como un material de desecho. Sin embargo, estudios recientes han demostrado su utilidad para diferentes propositos, como las aplicaciones biologicas. En el presente trabajo, se ha realizado un estudio para identificar los componentes principales de la membrana de la cascara del huevo y para determinar la posibilidad de usarlo como un biomaterial en ingenieria tisular. Para ello, se cultivaron fibroblastos en una membrana de cascara de huevo y luego se realizaron analisis histologicos e histoquimicos. Los resultados muestran no solo la presencia de colageno y proteoglicanos, sino tambien la viabilidad de las celulas dentro de la membrana. Estos resultados revelan el potencial de la membrana de la cascara del huevo para ser utilizado como biomaterial en ingenieria tisular. EnglishThe eggshell membrane has been long time considered as a waste material. However, recent studies have shown its usefulness for different purposes such as biological applications. In the present work, a study has been carried out to identify the eggshell membrane main components and to determine its feasibility for being used as a biomaterial in tissue engineering. Fibroblasts were cultured in eggshell membrane and then histological and histochemical analysis were performed. Results show not only the presence of collagen and proteoglycans, but also the viability of cells inside the membrane. These results revealed the eggshell membrane potential used as a biomaterial in tissue engineering.