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Cleaning of albumin-contaminated Ti and Cr surfaces: an XPS and QCM study
- Source :
- Surface and Interface Analysis. 40:215-219
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- The cleaning of protein-contaminated metallic surfaces is a major challenge for non-disposable devices used for biomedical applications and in the food industry. The objective of this work is to estimate the cleaning efficiency of sodium dodecyl sulfate (SDS) buffered at pH 7.4, as a function of temperature, for an immersion time of 15 h. Bovine serum albumin (BSA) is pre-adsorbed on pure Ti- and Cr-deposited quartz crystals. BSA adsorption and removal were investigated by X-ray photoelectron spectroscopy (XPS) and quartz crystal microbalance (QCM). Before cleaning, the amount of adsorbed BSA corresponds to one monolayer, and the oxide thickness is about 5 nm for the Cr quartz (Cr 3+ in oxide and hydroxide). For Ti quartz, the metal is not detected (10 nm or more of Ti 4+ in TiO 2 ). The C 1 s and N 1 s peaks indicate that the cleaning with SDS is achieved at 37 °C for Ti. For Cr, there are no significant changes from 37 to 60 °C. At 80 °C, the surface is partially cleaned (70% efficiency); at 90 °C, the surface cleaning with SDS is achieved (90% efficiency). The oxide layer of the chromium sample has not changed in composition and thickness. QCM measurements were performed at 37 °C. Switches between BSA and SDS solutions indicate that for Ti, SDS induces partial BSA removal (40% efficiency) after ∼20 min, while for Cr it seems that SDS adsorbs reversibly on the BSA layer, without any cleaning effect. Thus, QCM results are in agreement with XPS data obtained at the same temperature.
- Subjects :
- Materials science
Analytical chemistry
Oxide
chemistry.chemical_element
Surfaces and Interfaces
General Chemistry
Quartz crystal microbalance
Condensed Matter Physics
Surfaces, Coatings and Films
chemistry.chemical_compound
Chromium
Adsorption
X-ray photoelectron spectroscopy
chemistry
Monolayer
Materials Chemistry
Hydroxide
Sodium dodecyl sulfate
Subjects
Details
- ISSN :
- 10969918 and 01422421
- Volume :
- 40
- Database :
- OpenAIRE
- Journal :
- Surface and Interface Analysis
- Accession number :
- edsair.doi...........f64e886145f50db0472228e556f68395
- Full Text :
- https://doi.org/10.1002/sia.2655