A comparison of the effect of fucoidan from alga Fucus vesiculosus and its fractions obtained by anion-exchange chromatography on HeLa G-63, Hep G2, and Chang liver cells

The aim of this study was to compare the effects of sulfated fucopolysaccharides isolated from Fucus vesiculosus on HeLa G-63, Hep G2, and Chang liver cells. Native fucoidan F3 and two fractions (F3-0.5 and F3-1) obtained by anion-exchange chromatography were analyzed using chemical methods and IR spectroscopy. It was demonstrated that F3 and F3-1 are characterized by a higher content of sulfates, location of sulfo groups mostly at the C4 atom of fucose residue, and low content of uronic acids inhibited cell proliferation. Human liver carcinoma Hep G2 appeared to be the most sensitive to fucoidan, whereas nonmalignant human Chang liver cells were the least sensitive.