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Construction of the mutant strain in Aspergillus oryzae 3.042 for abundant proteinase production by the N+ ion implantation mutagenesis
- Source :
- International Journal of Food Science & Technology. 47:504-510
- Publication Year :
- 2011
- Publisher :
- Wiley, 2011.
-
Abstract
- Summary Because of secreting large amounts of enzymes, Aspergillus oryzae was widely used in the fermentation of soy sauce in many Asian countries. Here, N+ ion implantation mutagenesis was applied to improve the ability of A. oryzae to secrete acid protease. Several mutants were screened using three kinds of plates (Casein medium, Czapek’s medium and carboxymethyl cellulose medium agar plates). Compared with the other mutants, the mutant A100-8 could secrete the most protease. Acid protease activity of A100-8 was enhanced about 44.1% at 36 h by koji fermentation test. In addition, A100-8 was stable by periodically subculturing and maintaining on the agar slant. The mRNA expression levels of two kinds of acid protease (serine carboxypeptidase and aspartyl protease) were measured by RT-PCR at different periods. Serine carboxypeptidase and some kinds of aspartyl protease of A100-8 were significantly (P
- Subjects :
- chemistry.chemical_classification
Protease
medicine.medical_treatment
Mutant
Mutagenesis (molecular biology technique)
Biology
biology.organism_classification
Industrial and Manufacturing Engineering
Agar plate
Enzyme
chemistry
Biochemistry
Aspergillus oryzae
Casein
medicine
Fermentation
Food Science
Subjects
Details
- ISSN :
- 09505423
- Volume :
- 47
- Database :
- OpenAIRE
- Journal :
- International Journal of Food Science & Technology
- Accession number :
- edsair.doi...........f58e6875d40fd55fdefd6a2ec743ec03