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Design of a polyepitope construct for the induction of HLA-A0201-restricted HIV 1-specific CTL responses usingHLA-A*0201 transgenic,H-2 class I KO mice
- Source :
- European Journal of Immunology. 31:3064-3074
- Publication Year :
- 2001
- Publisher :
- Wiley, 2001.
-
Abstract
- HLA-A*0201 transgenic, H-2D(b)/mouse beta2-microglobulin double-knockout mice were used to compare and optimize the immunogenic potential of 17HIV 1-derived,HLA-A0201-restricted epitopic peptides. A tyrosine substitution in position 1 of the epitopic peptides, which increases both their affinity for and their HLA-A0201 molecule stabilizing capacity, was introduced in a significant proportion, having verified that such modifications enhance their immunogenicity in respect of their natural antigenicity. Based on these results, a 13-polyepitope construct was inserted in the pre-S2 segment of the hepatitis B middle glycoprotein and used for DNA immunization. Long-lasting CTL responses against most of the inserted epitopes could be elicited simultaneously in a single animal with cross-recognition in several cases of their most common natural variants.
Details
- ISSN :
- 15214141 and 00142980
- Volume :
- 31
- Database :
- OpenAIRE
- Journal :
- European Journal of Immunology
- Accession number :
- edsair.doi...........f4ee2e7939fc5da3b90fafee38894c53