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Development of polyclonal antibodies-based serological methods for detection of the rehmannia mosaic virus in field plants

Authors :
Jiahuan Chen
Chenwei Feng
Xiao Guo
Yuchen Zhou
Tianxiao Gu
Xinjian Zhuang
Lei Cheng
Kun Zhang
Source :
Frontiers in Sustainable Food Systems. 6
Publication Year :
2022
Publisher :
Frontiers Media SA, 2022.

Abstract

Rehmannia glutinosa is a top-grade traditional Chinese medicine, and also is an important planting medicinal material for Chinese poor farmers shaking off poverty. Rehmannia mosaic virus (ReMV) causes big economic loss of R. glutinosa in planting area. However, there is no effective methods for quick, accurate, and high-throughput detection for ReMV in Chinese production area. The preserved R. glutinosa samples carrying ReMV was taken for research material. The coat protein coding sequences (CPReMV) was cloned and sequenced. The target sequence was further placed into a prokaryotic expression vector to express the N-terminal-tagged recombinant CPReMV protein (His-CPReMV). Purified His-CPReMV was used as an antigen to immunize New Zealand white rabbits, and antiserum was obtained. The titers and sensitivities of the antisera were analyzed and evaluated. Polyclonal antibodies were purified from the antiserum, and the titers and sensitivity to the target His-CPReMV protein were evaluated. The results demonstrate that the obtained polyclonal antibodies against His-CPReMV could be successfully used for rapid, accurate, and high-throughput detection of ReMV from R. glutinosa planted in the wild. Our investigation established serological-based detection methods for ReMV for the first time, and provides a foundation for future exploration of the pathogenic mechanisms of ReMV in R. glutinosa.

Details

ISSN :
2571581X
Volume :
6
Database :
OpenAIRE
Journal :
Frontiers in Sustainable Food Systems
Accession number :
edsair.doi...........f4da8e6d4e45db7b32526fac88d0325f
Full Text :
https://doi.org/10.3389/fsufs.2022.1013470