Additional file 1: of Modulation of Wnt/BMP pathways during corneal differentiation of hPSC maintains ABCG2-positive LSC population that demonstrates increased regenerative potential

Full detailed descriptions for the hPSC differentiation towards LSCs and standard hPSC-LSC culture, establishment and maintenance of the ABCG2-positive hPSC-LSC culture, immunofluorescence characterization protocol, flow cytometry analysis and fluorescence-activated cell sorting protocol, quantitative RT-PCR protocol, and cell surface antigen screening with the LEGENDScreenâ ˘ Kit. (DOCX 29 kb)