Abstract WMP70: Etiology of Stroke Based on Early Analysis of Clot Cytometry Obtained Through First Pass Technique for Mechanical Thrombectomy

Introduction: Mechanical thrombectomy is the best treatment for large vessel occlusion in acute strokes, this technique can obtain clots for further analysis. Objective: To evaluate flow cytometry in thrombi obtained in the treatment of hyperacute stroke by mechanical thrombectomy, as a diagnostic tool in the etiological study of stroke Methods: Consecutively, intracranial clots were obtained in the hyperacute phase of stroke with solitaire device. Cell suspensions of thrombi were prepared that were labeled by direct immunofluorescence using conjugated monoclonal antibodies. The labeled samples were acquired in a Naviostm flow cytometer (Beckman-Coulter). The following leukocyte populations were studied: granulocytes, monocytes, total lymphocytes, T lymphocytes (CD3 +), helper T lymphocytes (CD3 +, CD4 +), suppressor-cytotoxic T lymphocytes (CD3 +, CD8 +), TNK lymphocytes (CD3 +, CD56 / 16 +) , NK lymphocytes (CD3-, CD56 / 16 +) and B lymphocytes (CD19 +). The results were expressed as percentages (%). The aetiology of stroke was categorized in secondary to: major structural heart disease, atrial fibrillation, stroke of atherosclerotic etiology (severe stenosis or complicated aortic atheromatosis ulceration) or infrequent causes. Results: 40 samples were analyzed. Clots of atherosclerotic etiology (n = 13) were associated with higher% of CD4 T lymphocytes (24.85% vs 15.83% p = 0.016), and higher% of NK (21.08% vs 17.04) % p = 0.07), also showed a tendency to a higher% LT (23.69% vs 16.46% p = 0.052). Strokes secondary to AF were associated with a higher percentage of CD8 T lymphocytes (20.24 vs 13.56 p = 0.048). Conclussion: Analysis by flow cytometry of clots obtained in the hyperacute phase of stroke showed significant differences in the different lymphocyte populations according to the etiology