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Inhibition of lipoxygenase-1 by tetrahydrocurcumin
- Source :
- European Food Research and Technology. 233:561-568
- Publication Year :
- 2011
- Publisher :
- Springer Science and Business Media LLC, 2011.
-
Abstract
- Tetrahydrocurcumin (THC)—the reduced form of curcumin—is more hydrophilic than its parent compound. It possesses higher stability in aqueous medium compared to curcumin. Lipoxygenase (LOX) enzyme is implicated in inflammatory conditions. THC was investigated for the inhibition of soy LOX-1. THC-inhibited LOX-1 with an IC50 value of 44.6 ± 0.6 μM. Kinetics of inhibition revealed mixed linear type with a Ki value of 46 μM. THC-inhibited LOX-1 by preventing the activation of LOX-1 as evidenced from spectroscopic studies. Molecular docking simulations suggested the binding of THC near the iron cofactor. This study helps in further understanding of the anti-inflammatory property of curcumin derivatives and provides valuable leads as an alternative of curcumin with better solubility and stability at physiological pH.
- Subjects :
- chemistry.chemical_classification
biology
Aqueous medium
Stereochemistry
organic chemicals
Kinetics
General Chemistry
Biochemistry
Industrial and Manufacturing Engineering
Cofactor
chemistry.chemical_compound
Lipoxygenase
Enzyme
chemistry
mental disorders
Curcumin
biology.protein
Solubility
IC50
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 14382385 and 14382377
- Volume :
- 233
- Database :
- OpenAIRE
- Journal :
- European Food Research and Technology
- Accession number :
- edsair.doi...........f3fc750875e7343da721c6e8ebf5b9ec