Studies on the Stabilization of Enzyme. II

Glycerol poly (oxyethylene) ether (GPEE) was found to effectively stabilize Candida cylindracea lipase in an aqueous solution (100U/ml) at 50°C. That is, this lipase became thermally stable in the presence of GPEE. Polyoxyethyleneglycol (PEG) and polypropyleneglycol (PPG) were also tested as stabilizing agents of this lipase in an aqueous solution and found to be effective.The relationship between the amount of additives in the lipase solution and their stabilizing extent was complicated. GPEE stabilized the lipase in proportion to its concentration and PEG brought about maximum stabilization at a concentration of 2.5mmol/20ml of solution. But stabilization became progressively less with increasing amount of PEG.Consequently, each additive is considered to have indivisual mechanism for imparting stability to a lipase solution.