CONTINUOUS GLUCOSE INFUSION AND ITS EFFECTS ON PULMONARY PHOSPHATIDYL CHOLINE (PC) AND DISATURATED PC (DSPC) IN FETAL RABBITS

It is known that infants of diabetic mothers(IDM) have a higher incidence of hyaline membrane disease(HMD). To determine the effect of continuous glucose infusion on PC and DSPC in lung tissue in vivo, a rabbit model was used. On 26th day of gestation, femoral vein was catheterized in a pregnant rabbit and D25 0.45% saline(group 1)or 0.9% saline(group 2)was infused over 46 to 48 hours. Sham operated pregnant rabbits underwent exposure of femoral vein and attempted catheterization(group 3). C-hysterectomy was performed on 28th day of gestation. Necks of the fetal rabbits were tied at delivery to prevent air breathing. Lungs were removed and analysed for PC and DSPC. Serum insulin and glucose were determined on fetal and doe's blood. Data on 6 rabbits in group 1, 4 rabbits in group 2 and 5 rabbits in group 3 are available. The serum insulin level in does in group 1 was higher compared to other two groups after 24 hours of infusion. The serum glucose in does after 24 hours in group 1 was higher than other two groups. As a % of total phospholipid(TPL), PC and DSPC were not different in all three groups.