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An Inexpensive Air Stream Temperature Controller and its use to facilitate temperature-controlled behavior in Drosophila

Publication Year :
2022
Publisher :
University of Virginia, 2022.

Abstract

Controlling the environment of an organism has many biologically relevant applications. Temperature-dependent inducible biological reagents have proven invaluable for elucidating signaling cascades and dissection of neural circuits. Here we develop a simple and affordable system for rapidly changing temperature in a chamber housing adult Drosophila melanogaster. Utilizing flies expressing the temperature-inducible channel dTrpA1 in dopaminergic neurons we show rapid and reproducible changes in locomotor behavior. This device should have wide application to temperature-modulated biological reagents.

Details

Database :
OpenAIRE
Accession number :
edsair.doi...........f2a4929721892c84ba9f9b8c56260f16
Full Text :
https://doi.org/10.18130/kedv-wx12