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An Inexpensive Air Stream Temperature Controller and its use to facilitate temperature-controlled behavior in Drosophila
- Publication Year :
- 2022
- Publisher :
- University of Virginia, 2022.
-
Abstract
- Controlling the environment of an organism has many biologically relevant applications. Temperature-dependent inducible biological reagents have proven invaluable for elucidating signaling cascades and dissection of neural circuits. Here we develop a simple and affordable system for rapidly changing temperature in a chamber housing adult Drosophila melanogaster. Utilizing flies expressing the temperature-inducible channel dTrpA1 in dopaminergic neurons we show rapid and reproducible changes in locomotor behavior. This device should have wide application to temperature-modulated biological reagents.
- Subjects :
- Inducible
Dopamine
Drosophila
Locomotor Behavior
TrpA1
Thermogenetics
Subjects
Details
- Database :
- OpenAIRE
- Accession number :
- edsair.doi...........f2a4929721892c84ba9f9b8c56260f16
- Full Text :
- https://doi.org/10.18130/kedv-wx12