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Genetic Diversity of Trichomonas vaginalis Clinical Isolates Determined by EcoRI Restriction Fragment Length Polymorphism of Heat-Shock Protein 70 Genes
- Source :
- The American Journal of Tropical Medicine and Hygiene. 80:245-251
- Publication Year :
- 2009
- Publisher :
- American Society of Tropical Medicine and Hygiene, 2009.
-
Abstract
- Restriction fragment length polymorphism (RFLP) analysis using a multilocus heat-inducible cytoplasmic heat-shock protein 70 (Hsp70) hybridization probe with EcoRI-digested genomic DNA was used in molecular typing of 129 Trichomonas vaginalis isolates. Results indicate that Trichomonas organisms exhibit considerable polymorphism in their Hsp70 RFLP patterns. Analysis of seven American Type Culture Collection reference strains and 122 clinical isolates, including 84 isolates from Jackson, Mississippi, 18 isolates from Atlanta, Georgia, and 20 isolates from throughout the United States, showed 105 distinct Hsp70 RFLP pattern subtypes for Trichomonas. Phylogenetic analysis of the Hsp70 RFLP data showed that the T. vaginalis isolates were organized into two clonal lineages. These results illustrate the substantial genomic diversity present in T. vaginalis and indicate that a large number of genetically distinct Trichomonas isolates may be responsible for human trichomoniasis in the United States.
- Subjects :
- Genetics
biology
Molecular epidemiology
Hybridization probe
Trichomonas
EcoRI
biology.organism_classification
medicine.disease_cause
genomic DNA
Infectious Diseases
Trichomonas Vaginitis
Virology
biology.protein
medicine
Parasitology
Trichomonas vaginalis
Restriction fragment length polymorphism
Subjects
Details
- ISSN :
- 14761645 and 00029637
- Volume :
- 80
- Database :
- OpenAIRE
- Journal :
- The American Journal of Tropical Medicine and Hygiene
- Accession number :
- edsair.doi...........f0ee67014039907cd6311a12d2ab3db8