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Trichostatin A and nuclear reprogramming of cloned rabbit embryos1
- Source :
- Journal of Animal Science. 86:1106-1113
- Publication Year :
- 2008
- Publisher :
- Oxford University Press (OUP), 2008.
-
Abstract
- To investigate the influence of histone deacetylases on nuclear reprogramming after nuclear transfer, we treated the cloned embryos with a histone deacetylase inhibitor, Trichostatin A (TSA). In the present study, global changes in acetylation of histone H3-lysine 14, histone H4-lysine 12, and histone H4-lysine 5 were studied in rabbit in vivo fertilized embryos, somatic cell nuclear transfer (SCNT) embryos, and TSA-treated SCNT embryos. From the pronuclear to the morula stage, the deacetylation-reacetylation changes in acetylation of histone H3-lysine 14 and histone H4-lysine 12 occurred in both fertilized embryos and TSA-treated cloned embryos; however, the distribution pattern in untreated cloned embryos failed to display such changes. More interesting, the signal of acetylation of histone H4-lysine 12 in cloned embryos was detected in both the inner cell mass and the trophectoderm, whereas TSA-treated cloned embryos showed the same staining pattern as fertilized embryos and the staining was limited to the inner cell mass. The histone acetylation pattern of TSA-treated SCNT embryos appeared to be more similar to that of normal embryos, indicating that TSA could improve nuclear reprogramming after nuclear transfer.
- Subjects :
- animal structures
biology
medicine.drug_class
Somatic cell
Histone deacetylase inhibitor
Embryo
General Medicine
complex mixtures
Molecular biology
Histone
Trichostatin A
Acetylation
embryonic structures
Genetics
biology.protein
medicine
bacteria
Somatic cell nuclear transfer
Inner cell mass
Animal Science and Zoology
sense organs
Food Science
medicine.drug
Subjects
Details
- ISSN :
- 15253163 and 00218812
- Volume :
- 86
- Database :
- OpenAIRE
- Journal :
- Journal of Animal Science
- Accession number :
- edsair.doi...........f0247f4591a817ec460037e0970c79af