Back to Search Start Over

Streptococcus agalactiae-induced autophagy of bovine mammary epithelial cell via PI3K/AKT/mTOR pathway

Authors :
Mengzhu Qi
Hao Geng
Na Geng
Yukun Cui
Changxi Qi
Guodong Cheng
Kaimin Song
Liping Hu
Yongxia Liu
Jianzhu Liu
Bo Han
Source :
Journal of Dairy Research. 89:178-184
Publication Year :
2022
Publisher :
Cambridge University Press (CUP), 2022.

Abstract

Streptococcus agalactiae (S. agalactiae) infection is a significant cause of mastitis, resulting in loss of cellular homeostasis and tissue damage. Autophagy plays an essential function in cell survival, defense, and the preservation of cellular homeostasis, and is often part of the response to pathogenic challenge. However, the effect of autophagy induced by S. agalactiae in bovine mammary epithelial cells (bMECs) is mainly unknown. So in this study, an intracellular S. agalactiae infection model was established. Through evaluating the autophagy-related indicators, we observed that after S. agalactiae infection, a significant quantity of LC3-I was converted to LC3-II, p62 was degraded, and levels of Beclin1 and Bcl2 increased significantly in bMECs, indicating that S. agalactiae induced autophagy. The increase in levels of LAMP2 and LysoTracker Deep Red fluorescent spots indicated that lysosomes had participated in the degradation of autophagic contents. After autophagy was activated by rapamycin (Rapa), the amount of p-Akt and p-mTOR decreased significantly, whilst the amount of intracellular S. agalactiae increased significantly. Whereas the autophagy was inhibited by 3-methyladenine (3MA), the number of intracellular pathogens decreased. In conclusion, the results demonstrated that S. agalactiae could induce autophagy through PI3K/Akt/mTOR pathway and utilize autophagy to survive in bMECs.

Details

ISSN :
14697629 and 00220299
Volume :
89
Database :
OpenAIRE
Journal :
Journal of Dairy Research
Accession number :
edsair.doi...........eff148ffcb492d01dbfaa8f40507abe6
Full Text :
https://doi.org/10.1017/s0022029922000243