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Antioxidant properties and global metabolite screening of the probiotic yeastSaccharomyces cerevisiaevar.boulardii
- Source :
- Journal of the Science of Food and Agriculture. 97:3039-3049
- Publication Year :
- 2017
- Publisher :
- Wiley, 2017.
-
Abstract
- Background: Saccharomyces cerevisiae var. boulardii is the only yeast species with probiotic properties. It is considered to have therapeutic significance in gastrointestinal disorders. In the present study, a comparative physiological study between this yeast and Saccharomyces cerevisiae (BY4742) was performed by evaluating two prominent traits of probiotic species, responses to different stress conditions and antioxidant capacity. A global metabolite profile was also developed aiming to identify which therapeutically important secondary metabolites are produced.; Results: Saccharomyces cerevisiae var. boulardii showed no significant difference in growth patterns but greater stress tolerance compared to S. cerevisiae. It also demonstrated a six- to 10-fold greater antioxidant potential (judged by the 1,1-diphenyl-2-picrylhydrazyl assay), with a 70-fold higher total phenolic content and a 20-fold higher total flavonoid content in the extracellular fraction. These features were clearly differentiated by principal component analysis and further indicated by metabolite profiling. The extracellular fraction of the S. cerevisiae var. boulardii cultures was found to be rich in polyphenolic metabolites: vanillic acid, cinnamic acid, phenyl ethyl alcohol (rose oil), erythromycin, amphetamine and vitamin B6 , which results in the antioxidant capacity of this strain.; Conclusion: The present study presents a new perspective for differentiating the two genetically related strains of yeast, S. cerevisiae and S. cerevisiae var. boulardii by assessing their metabolome fingerprints. In addition to the correlation of the phenotypic properties with the secretory metabolites of these two yeasts, the present study also emphasizes the potential to exploit S. cerevisiae var. boulardii in the industrial production of these metabolites. © 2016 Society of Chemical Industry.; © 2016 Society of Chemical Industry.
- Subjects :
- 0301 basic medicine
chemistry.chemical_classification
Nutrition and Dietetics
Antioxidant
biology
medicine.medical_treatment
Metabolite
030106 microbiology
Saccharomyces cerevisiae
Flavonoid
biology.organism_classification
Yeast
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
Biochemistry
chemistry
Polyphenol
medicine
Vanillic acid
Metabolome
Agronomy and Crop Science
Food Science
Biotechnology
Subjects
Details
- ISSN :
- 00225142
- Volume :
- 97
- Database :
- OpenAIRE
- Journal :
- Journal of the Science of Food and Agriculture
- Accession number :
- edsair.doi...........efee4bf92a4214469fe99c1aa9606b29
- Full Text :
- https://doi.org/10.1002/jsfa.8147