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A Rapid Aldosterone Radioimmunoassay1
- Source :
- The Journal of Clinical Endocrinology & Metabolism. 34:18-22
- Publication Year :
- 1972
- Publisher :
- The Endocrine Society, 1972.
-
Abstract
- This laboratory previously reported a radioimmunoassay (RIA) for aldosterone based on prior purification by solvent partitioning, periodate oxidation and a single thin-layer chromatogram. Other methods reported to date require paper chromatography to attain the required specificity. The method presented herein utilizes antibodies produced to aldosterone-γ-lactone 3-carboxymethyl oxime coupled to BSA. Aldosterone-γ-lactone constitutes the ligand. Overall interference is: cortisol 0.07%, corticosterone 0.02%, progesterone 0.17%, estradiol-ND, testosterone 0.02%, and 18-hydroxy corticosterone 0.29%. The purification procedure consists of CHCI3 extraction, benzene:H2O partition, periodate oxidation and bicarbonate wash. Recovery is determined from added 3H- or 14Caldosterone and is 40–50%. Simultaneous GC and RIA of urine samples (μg/24 hr) are: The correlation between GC and RIA methods is 0.95% (p < .01). RIA values with and without TLC showed no significant difference. This method is simple and reproducibl...
- Subjects :
- medicine.medical_specialty
Chromatography
Aldosterone
Endocrinology, Diabetes and Metabolism
Bicarbonate
Biochemistry (medical)
Clinical Biochemistry
Extraction (chemistry)
Periodate
Radioimmunoassay
Urine
Biochemistry
Paper chromatography
chemistry.chemical_compound
Endocrinology
chemistry
Corticosterone
Internal medicine
medicine
Subjects
Details
- ISSN :
- 19457197 and 0021972X
- Volume :
- 34
- Database :
- OpenAIRE
- Journal :
- The Journal of Clinical Endocrinology & Metabolism
- Accession number :
- edsair.doi...........ef36c7bb2d45dcbb496972a38ded98df