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Isolation of isoprene degrading bacteria from soils, development ofisoAgene probes and identification of the active isoprene-degrading soil community using DNA-stable isotope probing
- Source :
- Environmental Microbiology. 18:2743-2753
- Publication Year :
- 2016
- Publisher :
- Wiley, 2016.
-
Abstract
- Emissions of biogenic volatile organic compounds (bVOCs), are an important element in the global carbon cycle, accounting for a significant proportion of fixed carbon. They contribute directly and indirectly to global warming and climate change and have a major effect on atmospheric chemistry. Plants emit isoprene to the atmosphere in similar quantities to emissions of methane from all sources and each accounts for approximately one third of total VOCs. Although methanotrophs, capable of growth on methane, have been intensively studied, we know little of isoprene biodegradation. Here, we report the isolation of two isoprene-degrading strains from the terrestrial environment and describe the design and testing of polymerase chain reaction (PCR) primers targeting isoA, the gene encoding the active-site component of the conserved isoprene monooxygenase, which are capable of retrieving isoA sequences from isoprene-enriched environmental samples. Stable isotope probing experiments, using biosynthesized (13) C-labelled isoprene, identified the active isoprene-degrading bacteria in soil. This study identifies novel isoprene-degrading strains using both culture-dependent and, for the first time, culture-independent methods and provides the tools and foundations for continued investigation of the biogeography and molecular ecology of isoprene-degrading bacteria.
- Subjects :
- 0301 basic medicine
030106 microbiology
Stable-isotope probing
Biogeochemistry
Biodegradation
Biology
biology.organism_classification
Microbiology
Carbon cycle
03 medical and health sciences
chemistry.chemical_compound
030104 developmental biology
chemistry
Botany
Soil microbiology
Rhodococcus
Ecology, Evolution, Behavior and Systematics
Isoprene
Bacteria
Subjects
Details
- ISSN :
- 14622912
- Volume :
- 18
- Database :
- OpenAIRE
- Journal :
- Environmental Microbiology
- Accession number :
- edsair.doi...........eeccc85c407893222ba3f7caf1e2652d