Determination of Reaction Enthalpy and Conformational Volume Changes by Photoacoustic Spectroscopy

The reaction enthalpy and conformational volume changes can be qualitatively estimated by means of the temperature-dependent pulsed photoacoustic spectroscopy. The curves for heat-releasing fraction and conformational volume changes with different temperatures are presented. The simultaneous equations for determination of the enthalpy and volume changes are established based on temperature-dependent measurements, and applied to study the photolysis of coenzyme system.