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cDNA Cloning and Bioinformatic Analysis of Self-Incompatible S34-Allele from Chinese Pears
- Source :
- 2009 3rd International Conference on Bioinformatics and Biomedical Engineering.
- Publication Year :
- 2009
- Publisher :
- IEEE, 2009.
-
Abstract
- The full-length cDNA of a new S-RNase ( S 34 -RNase) (GenBank accession No.DQ414813) was successfully cloned from pistils of Chinese pear by RT-PCR and RACE methods. The S 34 -RNase shows a complete CDS of 687 nucleotides for putative 229 amino acids. The theoretical three-dimension structure of S 34 -RNase was predicted by homology modeling method and its structural characteristic was analyzed. Aligning of S 34 -RNase with other 39 S -RNases of pears revealed the question about unifying naming system.
Details
- Database :
- OpenAIRE
- Journal :
- 2009 3rd International Conference on Bioinformatics and Biomedical Engineering
- Accession number :
- edsair.doi...........ed39547b6358d905f288cfd31b778d34
- Full Text :
- https://doi.org/10.1109/icbbe.2009.5162479