Development of a LC-MS/MS Method for Simultaneous Determination of Bile Acids and their Conjugates in Hepatocytes, Tissue and Fluids in Rat

Background: It is still uncertain whether the accumulation of the bile acids (BAs) in hepatocytes beyond the normal stand after taking medicine which can cause hepatic injury or under the pathological state. Because it is lack of valid and simple analytical methods for the quantification of individual BAs and their taurine and glycine conjugates in the hepatocytes. It is necessary to develop methods for BAs quantification in hepatocytes to study their function in detail.