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Transcriptomic identification and expression analysis of cold shock domain protein (CSP) genes in the marine dinoflagellate Prorocentrum minimum
- Source :
- Journal of Applied Phycology. 33:843-854
- Publication Year :
- 2020
- Publisher :
- Springer Science and Business Media LLC, 2020.
-
Abstract
- Dinoflagellates are frequently exposed to cold-water temperatures, and some may survive in winter and in polar regions. Cold shock domain proteins (CSPs) seem to function as part of their adaptation to such stressful environments. In this study, we identified two CSP genes (PmCSP1 and PmCSP2) in the marine dinoflagellate Prorocentrum minimum, characterized their structural properties, and investigated their expression patterns in response to cold shock and heavy metal exposure. Putative PmCSP1 (96 aa, 10.1 kDa) and PmCSP2 (91 aa, 9.4 kDa) proteins had two consensus RNA-binding motifs and might be localized in the cytoplasm, due to the absence of a signal peptide. Phylogenetic analyses revealed that PmCSPs clustered with those of other dinoflagellates and had been recycled between genomic DNA and mRNA. Real-time PCR revealed that PmCSPs significantly responded to low temperatures, but not to nickel or cadmium. These genes may allow the organism to adapt to and survive in cold habitats and winter.
- Subjects :
- 0106 biological sciences
Signal peptide
biology
Phylogenetic tree
010604 marine biology & hydrobiology
Dinoflagellate
Plant Science
Aquatic Science
Cold-shock domain
biology.organism_classification
01 natural sciences
Transcriptome
genomic DNA
Biochemistry
Cytoplasm
Gene
010606 plant biology & botany
Subjects
Details
- ISSN :
- 15735176 and 09218971
- Volume :
- 33
- Database :
- OpenAIRE
- Journal :
- Journal of Applied Phycology
- Accession number :
- edsair.doi...........eb7c3f496f158f94b582bb43a03555d1
- Full Text :
- https://doi.org/10.1007/s10811-020-02332-9