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The Use of Lipid-coated Microbubbles as a Delivery Agent of 7/3-Hydroxycholesterol in a Radiofrequency Lesion in the Rat Brain
- Source :
- Neurosurgery. 42:592-598
- Publication Year :
- 1998
- Publisher :
- Ovid Technologies (Wolters Kluwer Health), 1998.
-
Abstract
- OBJECTIVE This laboratory has previously described the aggregation of intravenously administered lipid-coated microbubbles (LCM) around tumors and areas of injury. 7Beta-hydroxycholesterol has been used to inhibit astrocytic proliferation in nervous system injury models. The compound has been given by direct infusion, by epidural catheter, or in liposomes (delivered stereotactically to the injury site). In this article, we report the use of LCM to deliver 7beta-hydroxycholesterol to a radiofrequency injury site in the rat cerebrum. METHODS First, the ability of LCM to target the thermal lesion in the rat brain was characterized using a lipid-soluble fluorescent dye 3,3-dioctadecyloxacarbocyanine perchlorate. Then, the effectiveness of this delivery system in suppression of glial proliferation was measured by glial fibrillary acidic protein immunoreactivity. RESULTS Glial fibrillary acidic protein immunoreactivity was significantly reduced when 7beta-hydroxycholesterol was administered via LCM but not alone, suggesting that astrocytic proliferation would correspondingly be diminished. CONCLUSION LCM were assessed as a delivery vehicle for 7beta-hydroxycholesterol in a rat brain radiofrequency lesion and found to be efficient in reducing astrogliosis, as measured by glial fibrillary acidic protein immunoreactivity.
- Subjects :
- Nervous system
Liposome
Pathology
medicine.medical_specialty
Glial fibrillary acidic protein
biology
business.industry
Cerebrum
medicine.disease
Astrogliosis
Central nervous system disease
medicine.anatomical_structure
Injury Site
medicine
biology.protein
Microbubbles
Surgery
Neurology (clinical)
business
Subjects
Details
- ISSN :
- 15244040 and 0148396X
- Volume :
- 42
- Database :
- OpenAIRE
- Journal :
- Neurosurgery
- Accession number :
- edsair.doi...........eb5522f846851c5b0275e33d04c7d5ee