Additional file 3: Figure S3. of Rp58 and p27kip1 coordinate cell cycle exit and neuronal migration within the embryonic mouse cerebral cortex

The effects of Rp58 siRNA treatment together with expression constructs for p27kip1 and p27kip1ck- on progenitors. (A) There was a significant interaction between non-surface (SVZ) divisions in treated cells, identified as mitoses marked by pHH3 expression away from the ventricular surface (F3,9 = 7, p 600 cells counted from 3 to 4 independent brains per condition). (G-J) Representative photomicrographs of GFP and Tbr2 immunostained sections from each treatment, as indicated. (K) The proportion of Tbr2-expressing intermediate progenitors was significantly affected between treatment groups (F3,11 = 6.5, p = 0.00858, One-way ANOVA, >600 cells counted from 3 to 4 independent brains per condition). Values in graphs represent mean ± SEM. Scale bars represent 50 μm. (PDF 193 kb)